1G4I
Crystal structure of the bovine pancreatic phospholipase A2 at 0.97A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.532, 63.557, 37.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.970 |
| R-factor | 0.094 |
| Rwork | 0.094 |
| R-free | 0.11390 * |
| Starting model (for MR) | 1une |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.046 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.990 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.064 | 0.172 |
| Total number of observations | 469197 * | |
| Number of reflections | 66635 | |
| <I/σ(I)> | 33.4 | 6.89 |
| Completeness [%] | 99.8 | 97.4 |
| Redundancy | 7.04 | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.6 | 293 | MPD, calcium chloride, Tris buffer, pH 7.6, SMALL TUBES, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris | 100 (mM) | |
| 2 | 1 | 1 | 5 (mM) | ||
| 3 | 1 | 1 | protein | 30 (mg/ml) | |
| 4 | 1 | 2 | MPD |
