1G29
MALK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8424 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 190.718, 65.705, 77.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.260 - 1.900 |
| Rwork | 0.211 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.260 | 1.880 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.036 | 0.235 |
| Number of reflections | 82679 | |
| <I/σ(I)> | 24.6 | 4.5 |
| Completeness [%] | 99.0 | 93.8 |
| Redundancy | 4.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 18 * | 2M ammonium sulfate, 5% dioxane, 100mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | ADP | 1 (mM) | |
| 3 | 1 | drop | reservoir | ||
| 4 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 5 | 1 | reservoir | dioxane | 5 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
