1G17
CRYSTAL STRUCTURE OF SEC4-GUANOSINE-5'-(BETA,GAMMA)-IMIDOTRIPHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9802,0.9800,0.9537 |
Spacegroup name | P 65 |
Unit cell lengths | 84.504, 84.504, 86.469 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.700 - 2.000 |
R-factor | 0.264 |
Rwork | 0.264 |
R-free | 0.29200 |
RMSD bond length | 0.007 |
RMSD bond angle | 22.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.091 | 0.624 |
Total number of observations | 87371 * | |
Number of reflections | 23496 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 3.72 | 3.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 21 * | drop consists of equal amounts of protein and reservoir solutions * |
1 | Vapor diffusion, hanging drop * | 8 * | 21 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | 100 (mM) | ||
6 | 1 | reservoir | PEG4000 | 25 (%(v/v)) | |
7 | 1 | reservoir | ethylene glycol | 25-30 (%(v/v)) | |
8 | 1 | reservoir | 0.2 (M) | ||
9 | 1 | reservoir | sodium HEPES | 100 (mM) |