1G0W
CRYSTAL STRUCTURE OF BOVINE RETINAL CREATINE KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 95.672, 95.672, 107.913 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.300 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.23200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.222 |
| Number of reflections | 18575 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 96.8 * | 95.8 * |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 * | Loew, A., (1998) Acta Cryst., D54, 989. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | HEPES-HCl | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.86 (M) | |
| 4 | 1 | reservoir | PEG1000 | 1.8 (%) |
