1G0H

CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE-FRUCTOSE 1,6 BISPHOSPHATASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-08-27
Detector	UCSD MARK II
Spacegroup name	P 21 21 21
Unit cell lengths	65.180, 75.890, 131.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.300
R-factor	0.174 *
Rwork	0.224
R-free	0.23500 *
Starting model (for MR)	1awb
RMSD bond length	0.014
RMSD bond angle	0.021 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.069	0.411
Number of reflections	29087
<I/σ(I)>	12.2	1.1
Completeness [%]	98.7 *	95.9 *
Redundancy	3.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	PEG 8000, sodium chloride, calcium chloride, TRIS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	16 (mg/ml)
2	1	drop	PEG8000	11 (%(w/v))
3	1	drop	Tris-HCl	50 (mM)
4	1	drop		100 (mM)
5	1	drop		10 (mM)
6	1	reservoir	PEG8000	11-15 (%)