1G0H
CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE-FRUCTOSE 1,6 BISPHOSPHATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-08-27 |
| Detector | UCSD MARK II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.180, 75.890, 131.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.300 |
| R-factor | 0.174 * |
| Rwork | 0.224 |
| R-free | 0.23500 * |
| Starting model (for MR) | 1awb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.021 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.411 |
| Number of reflections | 29087 | |
| <I/σ(I)> | 12.2 | 1.1 |
| Completeness [%] | 98.7 * | 95.9 * |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | PEG 8000, sodium chloride, calcium chloride, TRIS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 16 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 11 (%(w/v)) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 11-15 (%) |
