1G0H
CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE-FRUCTOSE 1,6 BISPHOSPHATASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1997-08-27 |
Detector | UCSD MARK II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.180, 75.890, 131.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.300 |
R-factor | 0.174 * |
Rwork | 0.224 |
R-free | 0.23500 * |
Starting model (for MR) | 1awb |
RMSD bond length | 0.014 |
RMSD bond angle | 0.021 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 | 0.411 |
Number of reflections | 29087 | |
<I/σ(I)> | 12.2 | 1.1 |
Completeness [%] | 98.7 * | 95.9 * |
Redundancy | 3.6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | PEG 8000, sodium chloride, calcium chloride, TRIS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 16 (mg/ml) | |
2 | 1 | drop | PEG8000 | 11 (%(w/v)) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | 10 (mM) | ||
6 | 1 | reservoir | PEG8000 | 11-15 (%) |