1G0D
CRYSTAL STRUCTURE OF RED SEA BREAM TRANSGLUTAMINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-19 |
| Detector | FUJI |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 97.800, 97.800, 455.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.196 * |
| Rwork | 0.200 |
| R-free | 0.24900 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.454 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | 0.162 |
| Number of reflections | 39366 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 86.0 | 61.4 |
| Redundancy | 6.62 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | Ammonium sulfate, PEG6000, Hepes, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.8 (M) | |
| 2 | 1 | reservoir | PEG6000 | 1 (%) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | protein | 14 (mg/ml) |
