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THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). APO ENZYME.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-29 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 71.656, 73.656, 134.469 |
Unit cell angles | 89.98, 80.91, 80.91 |
Refinement procedure
Resolution | 73.000 - 1.900 |
R-factor | 0.18 |
Rwork | 0.176 |
R-free | 0.25100 |
RMSD bond length | 0.025 |
RMSD bond angle | 1.869 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.500 | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.054 | 0.286 |
Total number of observations | 377690 * | |
Number of reflections | 202988 | |
<I/σ(I)> | 9.2 | |
Completeness [%] | 96.1 | 95.6 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 * | Blankenfeldt, W., (2000) Acta Crystallogr.,Sect.D, 56, 1501. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 9-12 (%(w/v)) | |
2 | 1 | reservoir | lithium sulfate | 0.5 (M) | |
3 | 1 | reservoir | citrate/NaOH | 0.1 (M) |