1FZ5
METHANE MONOOXYGENASE HYDROXYLASE, FORM II CRYSTALLIZED ANAEROBICALLY FROM REDUCED ENZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.240, 171.570, 220.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.400 - 2.400 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.25300 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.470 |
Total number of observations | 907358 * | |
Number of reflections | 104366 | |
<I/σ(I)> | 12.5 | |
Completeness [%] | 97.9 | 97.3 |
Redundancy | 8.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8000, CaCl2, MOPS, 1,10-decanedioic acid. pH 7.0, VAPOR DIFFUSION, HANGING DROP at 298 K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 6 (%(w/w)) | |
10 | 1 | drop | sodium azide | 0.005 (%) | |
2 | 1 | reservoir | MOPS | 50 (mM) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | sodium azide | 0.01 (%) | |
5 | 1 | drop | MMOH | 25 (mM) | |
6 | 1 | drop | 1,10-decanedioic acid | 12.5 (%sat) | in H2O |
7 | 1 | drop | PEG8000 | 3 (%) | |
8 | 1 | drop | MOPS | 25 (mM) | |
9 | 1 | drop | 110 (mM) |