1FXX

THE STRUCTURE OF EXONUCLEASE I SUGGESTS HOW PROCESSIVITY IS ACHIEVED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1999-01-19
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	84.410, 84.410, 298.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.2202
Rwork	0.204
R-free	0.23600 *
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.050	0.267
Total number of observations	462433 *
Number of reflections	24672
<I/σ(I)>	14.4
Completeness [%]	95.9	96.2
Redundancy	5.9	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	1.85 - 2.0M Na/K Phosphate, 20% v/v Glycerol, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Na,K phosphate	1.85-2.0 (M)
2	1	reservoir	glycerol	20 (%(v/v))