1FXS
GDP-FUCOSE SYNTHETASE FROM ESCHERICHIA COLI COMPLEX WITH NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.200, 104.200, 75.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.000 - 2.300 |
| R-factor | 0.163 |
| R-free | 0.19200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | |
| Number of reflections | 20596 | |
| <I/σ(I)> | 26.4 | 9.1 |
| Completeness [%] | 97.4 | 81 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 4.0 M SODIUM FORMATE, pH 7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | sodium chloride | 50 (mM) | |
| 4 | 1 | reservoir | sodium formate | 4.0 (M) |
