1FX7
CRYSTAL STRUCTURE OF THE IRON-DEPENDENT REGULATOR (IDER) FROM MYCOBACTERIUM TUBERCULOSIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-12-01 |
Detector | APS-1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.309, 113.970, 236.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.27200 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.311 |
Total number of observations | 896284 * | |
Number of reflections | 82283 | |
<I/σ(I)> | 28.6 | |
Completeness [%] | 95.9 | 75.4 |
Redundancy | 10.9 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7 * | 4 * | 2.0 M lithium sulfate 0.01 M magnesium chloride 0.1 M MES buffer ~0.1 mM 21 bp duplex DNA oligomer, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 8-10 (mg/ml) | |
2 | 1 | 2 | 50 (mM) | ||
3 | 1 | 2 | Tris-HCl | 10 (mM) |