1FVE

X-RAY STRUCTURES OF THE ANTIGEN-BINDING DOMAINS FROM THREE VARIANTS OF HUMANIZED ANTI-P185-HER2 ANTIBODY 4D5 AND COMPARISON WITH MOLECULAR MODELING

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	39.200, 80.200, 86.100
Unit cell angles	113.10, 92.70, 102.60

Refinement procedure

Resolution	10.000 - 2.500 *
R-factor	0.171
Rwork	0.171
RMSD bond length	0.017
RMSD bond angle	3.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.084 *
Total number of observations	39775 *
Number of reflections	27387 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG3400	15 (%(w/v))
3	1	reservoir	imidazole malate	50 (mM)