1FVC

X-RAY STRUCTURES OF THE ANTIGEN-BINDING DOMAINS FROM THREE VARIANTS OF HUMANIZED ANTI-P185-HER2 ANTIBODY 4D5 AND COMPARISON WITH MOLECULAR MODELING

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	37.600, 63.400, 90.200
Unit cell angles	90.00, 98.20, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.183
Rwork	0.183
RMSD bond length	0.015
RMSD bond angle	3.200
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.116 *
Number of reflections	18259 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	imidazole malate	50 (mM)
2	1	reservoir	ammonium sulfate	32 (%sat)