1FTN
CRYSTAL STRUCTURE OF THE HUMAN RHOA/GDP COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-04 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.920, 65.960, 83.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.186 * |
| Rwork | 0.186 |
| R-free | 0.30400 |
| Structure solution method | MIR AND MR |
| Starting model (for MR) | POLY ALANINE MODEL OF RAC1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.048 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELX-90 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.080 |
| Rmerge | 0.045 | 0.121 |
| Total number of observations | 40036 * | |
| Number of reflections | 10913 | |
| <I/σ(I)> | 14.8 | 10.1 |
| Completeness [%] | 97.3 | 81.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7 (mg/ml) | |
| 10 | 1 | reservoir | PEG8000 | 28 (%) | |
| 11 | 1 | reservoir | Na citrate | 0.5 (mM) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 5.0 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | PEG400 | 10 (%) | |
| 6 | 1 | drop | GDP | 0.05 (mM) | |
| 7 | 1 | drop | dithiothreitol | 2.0 (mM) | |
| 8 | 1 | drop | 0.1 (%) | ||
| 9 | 1 | reservoir | Na acetate | 100 (mM) |
