1FS2

INSIGHTS INTO SCF UBIQUITIN LIGASES FROM THE STRUCTURE OF THE SKP1-SKP2 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	72.670, 87.020, 178.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.246
R-free	0.28700
RMSD bond length	0.012 *
RMSD bond angle	1.980 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.900 *
Rmerge	0.085 *	0.388 *
Total number of observations	178486 *
Number of reflections	23509 *
Completeness [%]	96.9 *	98.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	4 *	microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	16-18 (%)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir	dithiothreitol	5 (mM)