1FRP

CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH FRUCTOSE-2,6-BISPHOSPHATE, AMP AND ZN2+ AT 2.0 ANGSTROMS RESOLUTION. ASPECTS OF SYNERGISM BETWEEN INHIBITORS

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	60.950, 166.300, 80.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.189
Rwork	0.189
RMSD bond length	0.014
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.074 *
Total number of observations	115147 *
Number of reflections	49540
Completeness [%]	82.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4	20 *	pH 7.4, ROOM TEMPERATURE, COCRYSTALLIZATION.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-17 (mg/ml)
2	1	2	Tris-HCl	20 (mM)
3	1	2	AMP	1.0 (mM)
4	1	2	Fru-2,6-P2	0.2 (mM)
5	1	2		0.006 (mM)
6	1	2		5 (mM)
7	1	2	2-mercaptoethanol	1 (mM)
8	1	2	EDTA	0.1 (mM)
9	1	2	PEG3350	15 (%(w/v))