1FRO
HUMAN GLYOXALASE I WITH BENZYL-GLUTATHIONE INHIBITOR
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-20 |
| Detector | RIGAKU |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.000, 68.000, 169.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.500 - 2.200 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23400 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.700 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.248 |
| Total number of observations | 118633 * | |
| Number of reflections | 35503 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 91.3 | 79.1 * |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 15 * | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG2000 | 12.5-15 (%(w/v)) | |
| 2 | 1 | drop | MES | 25 (mM) | |
| 3 | 1 | drop | 0.05 (M) | ||
| 4 | 1 | drop | protein | 6 (mg/ml) | |
| 5 | 1 | drop | 2-mercaptoethanol | 0.5 (%) | |
| 6 | 1 | drop | S-benzyl-glutathione | 1 (mM) | |
| 7 | 1 | reservoir | PEG2000 | 25-30 (%(w/v)) | |
| 8 | 1 | reservoir | MES | 50 (mM) | |
| 9 | 1 | reservoir | 0.1 (M) |
