1FRG

CRYSTAL STRUCTURE, SEQUENCE, AND EPITOPE MAPPING OF A PEPTIDE COMPLEX OF AN ANTI-INFLUENZA HA PEPTIDE ANTIBODY FAB 26(SLASH)9: FINE-TUNING ANTIBODY SPECIFICITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	66.600, 115.100, 73.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.19
Rwork	0.190
RMSD bond length	0.015
RMSD bond angle	3.480
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.100 *	0.480 *
Total number of observations	45397 *
Number of reflections	15290 *	1237 *
Completeness [%]	87.0 *	43 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *		22.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab	5-10 (mg/ml)
2	1	reservoir	PEG10000	17 (%(w/v))
3	1	reservoir	imidazole malate	0.2 (M)
4	1	drop	peptide	0.8 (mg/ml)