1FPM
MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-15 |
Detector | RIGAKU RAXIS |
Spacegroup name | H 3 2 |
Unit cell lengths | 160.876, 160.876, 256.118 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
R-factor | 0.266 |
Rwork | 0.266 |
R-free | 0.31500 * |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.100 |
High resolution limit [Å] | 3.000 | 2.990 |
Rmerge | 0.088 | 0.182 |
Total number of observations | 137437 * | |
Number of reflections | 25025 | |
Completeness [%] | 97.0 | 94.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 | 295 | AMMONIUM SULFATE, PEG 1000, POTASSIUM MALEATE, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 46 (%sat) | |
2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
3 | 1 | reservoir | PEG1000 | 1 (%) | |
4 | 1 | reservoir | potassium maleate | 50 (mM) | pH7.6 |