1FOS
TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 241.100, 48.690, 66.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.050 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.32100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.090 * |
| Total number of observations | 76796 * |
| Number of reflections | 14316 |
| Completeness [%] | 91.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 20 * | pH 6.70, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | PEG 400 | ||
| 11 | 1 | 2 | NACL | ||
| 12 | 1 | 2 | MGCL2 | ||
| 13 | 1 | 2 | SPERMINE | ||
| 14 | 1 | 2 | BIS-TRIS-PROPANE | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | NH4 ACETATE | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | SPERMINE | ||
| 7 | 1 | 1 | DTT | ||
| 8 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 9 | 1 | 2 | WATER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fos63-jun63 | 0.5 (mM) | |
| 10 | 1 | reservoir | PEG400 | 11-15 (%) | |
| 11 | 1 | reservoir | 300 (mM) | ||
| 12 | 1 | reservoir | 55 (mM) | ||
| 13 | 1 | reservoir | spermine | 20. (mM) | |
| 14 | 1 | reservoir | dithiothreitol | 5-10 (mM) | |
| 15 | 1 | reservoir | bis-Tris | 50 (mM) | |
| 2 | 1 | drop | DNA | 0.6-0.8 (mM) | |
| 3 | 1 | drop | PEG400 | 5.5-7.5 (%) | |
| 4 | 1 | drop | ammonium acetate | 100-200 (mM) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | 27.5 (mM) | ||
| 7 | 1 | drop | spermine | 1.0 (mM) | |
| 8 | 1 | drop | dithiothreitol | 5-10 (mM) | |
| 9 | 1 | drop | bis-Tris | 25 (mM) |
