1FNS

CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN I546V MUTANT IN COMPLEX WITH THE FUNCTION BLOCKING FAB NMC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	90
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	209.396, 62.673, 73.789
Unit cell angles	90.00, 108.88, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.172
Rwork	0.172
R-free	0.20700
RMSD bond length	0.018
RMSD bond angle	26.500 *
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *
Rmerge	0.076 *	0.408 *
Total number of observations	175343 *
Number of reflections	57228 *
Completeness [%]	93.2 *	82.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	calcium acetate	60 (mM)
3	1	reservoir	mPEG5000	12 (%(w/v))
4	1	reservoir	Tris-HCl	0.12 (M)