1FN9
CRYSTAL STRUCTURE OF THE REOVIRUS OUTER CAPSID PROTEIN SIGMA 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 198 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.898, 82.564, 132.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 1.800 |
R-factor | 0.169 * |
Rwork | 0.169 |
R-free | 0.19500 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.540 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.107 |
Number of reflections | 79737 | |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.1 | 96 |
Redundancy | 5.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 4 * | PEG 6000, sodium chloride, magnesium chloride, tris-HCl, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 302K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 4 * | PEG 6000, sodium chloride, magnesium chloride, tris-HCl, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 302K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 4 * | PEG 6000, sodium chloride, magnesium chloride, tris-HCl, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 302K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
10 | 1 | reservoir | PEG6000 | 1-3 (%) | |
2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 800 (mM) | ||
5 | 1 | drop | Tris-HCl | 50 (mM) | pH8.3 |
6 | 1 | drop | PEG6000 | 1-3 (%) | |
7 | 1 | reservoir | Tris | 10 (mM) | |
8 | 1 | reservoir | 10 (mM) | ||
9 | 1 | reservoir | Tris-HCl | 50 (mM) |