1FLC
X-RAY STRUCTURE OF THE HAEMAGGLUTININ-ESTERASE-FUSION GLYCOPROTEIN OF INFLUENZA C VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 155.400, 155.400, 414.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26700 |
| Structure solution method | SIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.54) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 10.000 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 * | 0.100 |
| Number of reflections | 80900 | |
| <I/σ(I)> | 12 | 12 |
| Completeness [%] | 99.0 | 91 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.1 | pH 7.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 10 | 1 | reservoir | 0.1 (%) | ||
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 140 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 0.1 (%) | ||
| 6 | 1 | reservoir | ammonium sulfate | 56-60 (%sat) | |
| 7 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 8 | 1 | reservoir | 140 (mM) | ||
| 9 | 1 | reservoir | 10 (mM) |
