1FKP
CRYSTAL STRUCTURE OF NNRTI RESISTANT K103N MUTANT HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH NEVIRAPINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-02 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 139.700, 109.800, 72.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.209 |
| Rwork | 0.215 |
| R-free | 0.28100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.151 | 0.571 |
| Total number of observations | 81694 * | |
| Number of reflections | 24586 | 2146 * |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 94.9 | 84.5 |
| Redundancy | 3.32 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 4 * | Ren, J., (1998) Proc.Natl.Acad.Sci.USA, 95, 9518. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 26 (mg/ml) | |
| 2 | 1 | drop | PEG3400 | 6 (%(w/v)) | |
| 3 | 1 | drop | citrate/phosphate | ||
| 4 | 1 | reservoir | PEG | 6 (%(w/v)) |
