1FIU
TETRAMERIC RESTRICTION ENDONUCLEASE NGOMIV IN COMPLEX WITH CLEAVED DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.400, 91.130, 149.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.260 - 1.600 |
| R-factor | 0.174 * |
| Rwork | 0.173 |
| R-free | 0.20380 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.567 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.260 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | |
| Total number of observations | 2553379 * | |
| Number of reflections | 160528 | |
| Completeness [%] | 98.6 | 88.7 |
| Redundancy | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 291 | MES 100mM, MgCl2 200mM, MPD 25%, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MES | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 25 (%(v/w)) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 4 | 1 | drop | protein | 0.2 (mM) | |
| 5 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 6 | 1 | drop | 200 (mM) | ||
| 7 | 1 | drop | 0.02 (%(w/v)) |
