1FIT
FHIT (FRAGILE HISTIDINE TRIAD PROTEIN)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-05 |
| Detector | FUJI |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.600, 50.600, 268.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.22500 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 25.030 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.056 |
| Total number of observations | 197773 * |
| Number of reflections | 35339 |
| Completeness [%] | 94.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 1.25-1.35M AMSO4, 100MM SODIUM CACODYLATE PH6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | AmSO4 | 1.25-1.35 (M) | |
| 3 | 1 | 1 | sodium cacodylate | 100 (mM) |
