1FHF
THE STRUCTURE OF SOYBEAN PEROXIDASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2000-11-24 |
Detector | MARRESEARCH |
Spacegroup name | P 31 |
Unit cell lengths | 106.454, 106.454, 104.996 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 11.000 - 2.800 |
R-factor | 0.271 |
Rwork | 0.271 |
R-free | 0.28900 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.096 | 0.289 |
Total number of observations | 175095 * | |
Number of reflections | 26929 | |
<I/σ(I)> | 7 | |
Completeness [%] | 88.2 | 69.3 |
Redundancy | 6.5 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M tris hcl, 0.6M ammoniumdihydrogenphosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | protein | 1.9 (mg/ml) | |
3 | 1 | reservoir | 0.2-0.6 (M) | ||
4 | 1 | reservoir | Tris-HCl | 0.1 (M) |