1FH2

TRANSTHYRETIN STABILITY AS A KEY FACTOR IN AMYLOIDOGENESIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-11-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	43.770, 86.310, 65.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.1667
Rwork	0.170
R-free	0.21280
RMSD bond length	0.012
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.820
High resolution limit [Å]	1.800	1.800
Rmerge	0.057	0.318
Total number of observations	121001 *
Number of reflections	23459 *
<I/σ(I)>	20
Completeness [%]	100.0	100
Redundancy	5.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.3 *	287	Damas, A.M., (1996) Acta Crystallog., D52, 966. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	41 (%)
3	1	reservoir	sodium citrate	200 (mM)