1FFY
INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | PRINCETON 2K |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.000, 100.000, 186.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| Rwork | 0.239 |
| R-free | 0.28100 |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 500.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.079 |
| Number of reflections | 67758 |
| Completeness [%] | 89.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 20 * | This particular structure is not described in this paper. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 10 | 1 | 2 | PEG 6000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | MGSO4 | ||
| 4 | 1 | 1 | KCL | ||
| 5 | 1 | 1 | ZnCl2 | ||
| 6 | 1 | 1 | BETA OCTYL GLUCOPYRANOSIDE | ||
| 7 | 1 | 1 | BETA MERCAPTOETHANOL | ||
| 8 | 1 | 1 | BICINE | ||
| 9 | 1 | 1 | PEG 6000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | IleRS | 0.050 (mM) | |
| 2 | 1 | drop | tRNA | 0.050 (mM) | |
| 3 | 1 | drop | Mupirocin | 1 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 12 (%) | |
| 5 | 1 | reservoir | 0.3 (M) | ||
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | 2 (mM) | ||
| 9 | 1 | reservoir | beta-octyl glucopyranoside | 0.1 (%) |
