1FFK
CRYSTAL STRUCTURE OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI AT 2.4 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.660, 299.670, 573.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.000 - 2.400 |
| R-factor | 0.252 * |
| Rwork | 0.252 |
| R-free | 0.26100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 28.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.691 |
| Total number of observations | 6089802 * | |
| Number of reflections | 666819 | |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 95.6 | 71 * |
| Redundancy | 9.1 | 6.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | back-extraction, vapor diffusion * | 7.1 * | 292 | PEG 6000, KCl, NH4Cl, MgCl2, CdCl2, potassium acetate, Tris-MES, pH 5.8, backextraction, vapor diffusion, temperature 292K |
| 1 | back-extraction, vapor diffusion * | 7.1 * | 292 | PEG 6000, KCl, NH4Cl, MgCl2, CdCl2, potassium acetate, Tris-MES, pH 5.8, backextraction, vapor diffusion, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | CdCl2 | ||
| 5 | 1 | 1 | potassium acetate | ||
| 6 | 1 | 1 | Tris-MES | ||
| 7 | 1 | 1 | PEG 6000 | ||
| 8 | 1 | 2 | PEG 6000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.2 (M) | ||
| 2 | 1 | reservoir | 0.5 (M) | ||
| 3 | 1 | reservoir | Kacetate | 100 (mM) | |
| 4 | 1 | reservoir | 30 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 7 (%) | |
| 6 | 1 | reservoir | Tris | 15 (mM) | |
| 7 | 1 | reservoir | MES | 15 (mM) | |
| 8 | 1 | reservoir | 1 (mM) |
