1FCV
CRYSTAL STRUCTURE OF BEE VENOM HYALURONIDASE IN COMPLEX WITH HYALURONIC ACID TETRAMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.012, 71.012, 151.326 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.650 |
| R-factor | 0.18 |
| Rwork | 0.163 |
| R-free | 0.25200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.295 |
| Number of reflections | 13411 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 20 * | PEG 4000, ammonium sulphate, Na acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 5 (mM) | |
| 2 | 1 | drop | protein | 8-10 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 30 (%) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
