1FCM

CRYSTAL STRUCTURE OF THE E.COLI AMPC BETA-LACTAMASE MUTANT Q120L/Y150E COVALENTLY ACYLATED WITH THE INHIBITORY BETA-LACTAM, CLOXACILLIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	106
Spacegroup name	C 1 2 1
Unit cell lengths	118.520, 76.190, 98.280
Unit cell angles	90.00, 116.68, 90.00

Refinement procedure

Resolution	20.000 - 2.460
Rwork	0.207
R-free	0.26500
RMSD bond length	0.015 *
RMSD bond angle	1.900 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.460
Rmerge	0.121 *	0.284 *
Number of reflections	26662 *
<I/σ(I)>	12.4
Completeness [%]	92.8 *	88.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	23 *	microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.1 (mM)
2	1	reservoir	potassium phosphate	1.7 (M)