1FBA

THE CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM DROSOPHILA MELANOGASTER AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	86.600, 116.800, 151.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.179
RMSD bond length	0.010
RMSD bond angle	0.021 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.097 *
Total number of observations	377901 *
Number of reflections	123654 *
Completeness [%]	87.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	2-4 *	referred to J.Biol.Chem. 257.11747-11749 1987 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	10 (%)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	beta-mercaptoethanol	1 (mM)
5	1	drop	dithioerythritol	0.1 (mM)
6	1	drop	protein	7.5 (mg/ml)
7	1	reservoir	PEG6000	20 (%)
8	1	reservoir	Tris-HCl	100 (mM)