1FAP

THE STRUCTURE OF THE IMMUNOPHILIN-IMMUNOSUPPRESSANT FKBP12-RAPAMYCIN COMPLEX INTERACTING WITH HUMAN FRAP

Experimental procedure

Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-07-29
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	44.630, 52.140, 102.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.193
Rwork	0.193
R-free	0.29900
RMSD bond length	0.008
RMSD bond angle	1.480
Data reduction software	UCSD
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.700
Rmerge	0.071
Total number of observations	43447 *
Number of reflections	6920
Completeness [%]	98.5
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	FKBP12	10 (mg/ml)
2	1	drop	Tris-HCl	60 (mM)
3	1	drop	rapamycin
4	1	drop	methanol
5	1	drop	FRB	10 (mg/ml)
6	1	reservoir	PEG8000	200 (%(w/v))
7	1	reservoir	methylpentanediol	10 (%)
8	1	reservoir	Tris-HCl	10 (mM)