1FAK
HUMAN TISSUE FACTOR COMPLEXED WITH COAGULATION FACTOR VIIA INHIBITED WITH A BPTI-MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.490, 190.000, 175.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.100 |
| R-factor | 0.237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.041 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.100 | 2.100 * |
| Rmerge | 0.094 | |
| Total number of observations | 166318 * | |
| Number of reflections | 70671 * | 61209 * |
| Completeness [%] | 87.0 | 78 * |
| Redundancy | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 10 | 1 | drop | 1-propanol | 3 (%(v/v)) | |
| 2 | 1 | reservoir | PEG4000 | 16.0-16.5 (%(w/v)) | |
| 3 | 1 | reservoir | 1-propanol | 12 (%(v/v)) | |
| 4 | 1 | drop | protein | 3.4 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 15 (mM) | |
| 6 | 1 | drop | sodium citrate | 25 (mM) | |
| 7 | 1 | drop | 37.5 (mM) | ||
| 8 | 1 | drop | 1.5 (mM) | ||
| 9 | 1 | drop | PEG4000 | 4 (%(w/v)) |
