1F9V

CRYSTAL STRUCTURES OF MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE KINESIN MOTOR ATPASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-20
Spacegroup name	P 1 21 1
Unit cell lengths	43.940, 77.370, 47.710
Unit cell angles	90.00, 105.88, 90.00

Refinement procedure

Resolution	20.000 - 1.300
R-factor	0.218 *
Rwork	0.218
R-free	0.24220
RMSD bond length	0.006
RMSD bond angle	1.215
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.046
Total number of observations	929216 *
Number of reflections	70750
<I/σ(I)>	41.7
Completeness [%]	94.0	87.8
Redundancy	13.1	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	HEPES, PEG 2000 ME, Ethylen glycol,magnesium chloride, Sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG2000 ME	20-26 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	HEPES	50 (mM)