1F9I

CRYSTAL STRUCTURE OF THE PHOTOACTIVE YELLOW PROTEIN MUTANT Y42F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-03-04
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	65.699, 65.699, 40.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.100
R-factor	0.127 *
Rwork	0.127
R-free	0.17100
RMSD bond length	0.014
RMSD bond angle	0.035
Data reduction software	XFIT
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.130
High resolution limit [Å]	1.100	1.100
Rmerge	0.098	0.390 *
Total number of observations	186098 *
Number of reflections	40647
<I/σ(I)>	24
Completeness [%]	99.5	99
Redundancy	4.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	292	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.5 (M)
2	1	reservoir	HEPES	20 (mM)