1F8C

Native Influenza Neuraminidase in Complex with 4-amino-2-deoxy-2,3-dehydro-N-neuraminic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS II
Spacegroup name	I 4 3 2
Unit cell lengths	180.980, 180.980, 180.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
Rwork	0.178
R-free	0.21600
RMSD bond length	0.012
RMSD bond angle	1.780
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.096	0.550
Total number of observations	409163 *
Number of reflections	47677
<I/σ(I)>	11.3
Completeness [%]	86.1	33.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.9	293	Laver, W.G., (1984) Virology, 137, 314. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	1.7 (M)
2	1	drop	protein	10-15 (mg/ml)
3	1	reservoir	potassium phosphate	1.9 (M)