1F7T
HOLO-(ACYL CARRIER PROTEIN) SYNTHASE AT 1.8A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.262, 76.160, 85.693 |
| Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.760 | 0.461 |
| Total number of observations | 314385 * | |
| Number of reflections | 87902 * | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 96.4 | 68.6 |
| Redundancy | 3.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 291 | Protein: 10mM Sodium Acetate pH 4.4, 2mM Magnesium chloride, 100mM sodium chloride, 5mM Dithiothreitol Well: 2.5M Sodium chloride, 0.1M Tris pH 7.0, 0.2M Magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
| 1 | unknown * | 7 | 291 | Protein: 10mM Sodium Acetate pH 4.4, 2mM Magnesium chloride, 100mM sodium chloride, 5mM Dithiothreitol Well: 2.5M Sodium chloride, 0.1M Tris pH 7.0, 0.2M Magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 2.5 (M) | ||
| 2 | 1 | 1 | Tris | 0.1 (M) | |
| 3 | 1 | 1 | 0.2 (M) |
