1F6Y
MAD CRYSTAL STRUCTURE ANALYSIS OF METHYLTETRAHYDROFOLATE: CORRINOID/IRON-SULFUR PROTEIN METHYLTRANSFERASE (METR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.000, 89.412, 115.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.201 |
| R-free | 0.23060 * |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.000 |
| RMSD bond angle | 0.000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.294 |
| Number of reflections | 28189 | |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 81.8 | 87.6 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 298 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 2 (mM) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | reservoir | PEGmme5000 | 9-15 (%(w/v)) | |
| 6 | 1 | reservoir | 20-50 (mM) | ||
| 7 | 1 | reservoir | glycerol | 20 (%) | |
| 8 | 1 | reservoir | HEPES | 50 (mM) |
