1F6A

Structure of the human ige-fc bound to its high affinity receptor fc(epsilon)ri(alpha)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	113
Detector technology	CCD
Collection date	1999-11-04
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	192.800, 192.800, 302.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.870 - 3.500
R-factor	0.254
Rwork	0.254
R-free	0.27300
RMSD bond length	0.010
RMSD bond angle	25.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.091	0.461
Total number of observations	84873 *
Number of reflections	27484 *
<I/σ(I)>	11
Completeness [%]	99.8	99.7
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	23 *	Ammonium Sulfate, Tris, CHAPS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	8.5	23 *	Ammonium Sulfate, Tris, CHAPS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	ammonium sulfate	1.4-1.6 (M)
4	1	reservoir	CHAPS	8 (mM)