1F66

2.6 A CRYSTAL STRUCTURE OF A NUCLEOSOME CORE PARTICLE CONTAINING THE VARIANT HISTONE H2A.Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-29
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	105.660, 183.207, 109.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.600
R-factor	0.193 *
Rwork	0.193
R-free	0.24950
RMSD bond length	0.013 *
RMSD bond angle	1.550 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.112	0.245
Number of reflections	65959
<I/σ(I)>	7.7
Completeness [%]	99.6	99.9
Redundancy	4.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	292	used macroseeding *
1	Vapor diffusion *	6	292	used macroseeding *
1	Vapor diffusion *	6	292	used macroseeding *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MnCl2
2	1	1	KCl
3	1	1	cacodylate
4	1	2	KCl

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		80-96 (mM)
2	1	drop		68-78 (mM)
3	1	drop	potassium cacodylate	20 (mM)
4	1	drop	protein	4 (mg/ml)
5	1	reservoir		40-48 (mM)
6	1	reservoir		34-39 (mM)
7	1	reservoir	potassium cacodylate	20 (mM)