1F66
2.6 A CRYSTAL STRUCTURE OF A NUCLEOSOME CORE PARTICLE CONTAINING THE VARIANT HISTONE H2A.Z
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-29 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.660, 183.207, 109.922 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.600 |
R-factor | 0.193 * |
Rwork | 0.193 |
R-free | 0.24950 |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.550 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.112 | 0.245 |
Number of reflections | 65959 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 4.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MnCl2 | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | cacodylate | ||
4 | 1 | 2 | KCl |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 80-96 (mM) | ||
2 | 1 | drop | 68-78 (mM) | ||
3 | 1 | drop | potassium cacodylate | 20 (mM) | |
4 | 1 | drop | protein | 4 (mg/ml) | |
5 | 1 | reservoir | 40-48 (mM) | ||
6 | 1 | reservoir | 34-39 (mM) | ||
7 | 1 | reservoir | potassium cacodylate | 20 (mM) |