1F66
2.6 A CRYSTAL STRUCTURE OF A NUCLEOSOME CORE PARTICLE CONTAINING THE VARIANT HISTONE H2A.Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-29 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.660, 183.207, 109.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.193 * |
| Rwork | 0.193 |
| R-free | 0.24950 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.550 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.112 | 0.245 |
| Number of reflections | 65959 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
| 1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
| 1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MnCl2 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 2 | KCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 80-96 (mM) | ||
| 2 | 1 | drop | 68-78 (mM) | ||
| 3 | 1 | drop | potassium cacodylate | 20 (mM) | |
| 4 | 1 | drop | protein | 4 (mg/ml) | |
| 5 | 1 | reservoir | 40-48 (mM) | ||
| 6 | 1 | reservoir | 34-39 (mM) | ||
| 7 | 1 | reservoir | potassium cacodylate | 20 (mM) |
