1F52
CRYSTAL STRUCTURE OF GLUTAMINE SYNTHETASE FROM SALMONELLA TYPHIMURIUM CO-CRYSTALLIZED WITH ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-24 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.600, 132.500, 195.900 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 34.900 - 2.490 |
| R-factor | 0.2434 |
| Rwork | 0.243 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.950 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 2.530 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.082 | 0.133 |
| Number of reflections | 196561 | |
| <I/σ(I)> | 32.6 | |
| Completeness [%] | 98.2 | 87.8 |
| Redundancy | 10 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | ADP, MPD, spermine, manganese chloride, imidazole buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | imidazole/HCl | 15 (mM) | |
| 2 | 1 | 1 | 3 (mM) | ||
| 3 | 1 | 1 | spermine tetrahydrochloride | 3 (mM) | |
| 4 | 1 | 1 | MPD | 10 (%) |
