1F30
THE STRUCTURAL BASIS FOR DNA PROTECTION BY E. COLI DPS PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 95 |
Detector technology | AREA DETECTOR |
Collection date | 1998-06-20 |
Detector | SDMS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.773, 140.780, 268.419 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.470 - 2.850 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DPS D75C D78A UNPUBLISHED STRUCTURE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.3) |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.850 |
Rmerge | 0.067 | 0.235 |
Number of reflections | 51548 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.0 | 99.3 |
Redundancy | 3.97 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10mM MOPS, 100mM KCl, 10% glycerol + 100mM TrisHCL, 100mM KCl, 10% glycerol, 11% PEG 8000, and 5mM DTT, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |