1F30

THE STRUCTURAL BASIS FOR DNA PROTECTION BY E. COLI DPS PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	95
Detector technology	AREA DETECTOR
Collection date	1998-06-20
Detector	SDMS
Spacegroup name	C 2 2 21
Unit cell lengths	117.773, 140.780, 268.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.470 - 2.850
R-factor	0.232
Rwork	0.232
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DPS D75C D78A UNPUBLISHED STRUCTURE
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.3)
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.900
High resolution limit [Å]	2.800	2.850
Rmerge	0.067	0.235
Number of reflections	51548
<I/σ(I)>	13.3
Completeness [%]	99.0	99.3
Redundancy	3.97	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	298	10mM MOPS, 100mM KCl, 10% glycerol + 100mM TrisHCL, 100mM KCl, 10% glycerol, 11% PEG 8000, and 5mM DTT, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K