1F2X
STRUCTURE OF THE SINGLE-DOMAIN CAMELID ANTIBODY CAB-CA05
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-05 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.980, 43.860, 87.950 |
Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
Resolution | 31.000 - 2.100 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bzq |
RMSD bond length | 0.009 |
RMSD bond angle | 0.027 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.103 | 0.227 |
Number of reflections | 13256 | |
<I/σ(I)> | 4.9 | 2.7 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 * | 293 | Desmyter, A., (1996) Nature Struct. Biol., 3, 803. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein complex | 5 (mg/ml) | |
2 | 1 | drop | PEG8000 | 15-20 (%(w/v)) | |
3 | 1 | drop | potassium phosphate | 0.1 (M) |