1F0C
STRUCTURE OF THE VIRAL SERPIN CRMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-23 |
Detector | BRANDEIS - B4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.203, 92.455, 100.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.260 |
R-factor | 0.2277 * |
Rwork | 0.228 |
R-free | 0.27200 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.720 |
Data reduction software | MADNESS |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.310 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.051 | 0.281 |
Total number of observations | 75617 * | |
Number of reflections | 21897 | |
<I/σ(I)> | 26.3 | |
Completeness [%] | 96.2 | 94.7 * |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5 * | 23 * | 0.1 M Na-Acetate, 15% PEG 4000, 0.1 M MgCl2, VAPOR DIFFUSION, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-10 (mg/ml) | |
2 | 1 | drop | sodium acetate | 0.1 (M) | |
3 | 1 | drop | PEG4000 | 20 (%) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
5 | 1 | reservoir | PEG4000 | 15 (%) | |
6 | 1 | reservoir | 0.1 (M) |