1F07
STRUCTURE OF COENZYME F420 DEPENDENT TETRAHYDROMETHANOPTERIN REDUCTASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-07 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.800, 128.200, 109.200 |
Unit cell angles | 90.00, 131.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.23700 |
RMSD bond length | 0.008 |
RMSD bond angle | 21.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.290 |
Number of reflections | 107409 | |
<I/σ(I)> | 14 | |
Completeness [%] | 95.0 | 88 |
Redundancy | 3.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 277 | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 30 (%) | |
2 | 1 | reservoir | 0.1 (M) | ||
3 | 1 | reservoir | 0.02 (M) |