1EZV
STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 1999-02-01 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 214.470, 163.920, 147.270 |
Unit cell angles | 90.00, 117.50, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.253 |
Rwork | 0.222 |
R-free | 0.25400 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 15.000 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.065 | 0.390 |
Total number of observations | 1057968 * | |
Number of reflections | 168625 * | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 84.9 | 73.9 |
Redundancy | 6.27 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | 277 | 5 % PEG 4000, 100 mM Tris, 0.05 % Undecyl-maltoside, 1 micromolar stigmatellin, pH 8.0, microseeding, temperature 277K |
1 | Vapor diffusion * | 8 | 277 | 5 % PEG 4000, 100 mM Tris, 0.05 % Undecyl-maltoside, 1 micromolar stigmatellin, pH 8.0, microseeding, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | ||
2 | 1 | drop | PEG4000 | ||
3 | 1 | drop | Tris-HCl | 100 (mM) | |
4 | 1 | drop | UDM | 0.05 (%) | |
5 | 1 | reservoir | PEG4000 | 5-6 (%) |