1EZJ
CRYSTAL STRUCTURE OF THE MULTIMERIZATION DOMAIN OF THE PHOSPHOPROTEIN FROM SENDAI VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 48.584, 48.584, 100.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.255 |
| Rwork | 0.249 |
| R-free | 0.29300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.089 | 0.386 |
| Number of reflections | 9175 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 92.1 | 94.5 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | PEG 3000 16%, Sodium chloride 200 mM, Calcium chloride 10 mM, ethylmercurythiosalicilic acid 5 mM, Tris-HCl 100 mM, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | PEG3000 | 15-17 (%(w/v)) | |
| 8 | 1 | reservoir | EMTS | 5 (mM) |
