1EYR

Structure of a sialic acid activating synthetase, CMP acylneuraminate synthetase in the presence and absence of CDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-16
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	43.010, 69.890, 157.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.200
R-factor	0.185
Rwork	0.185
R-free	0.24700 *
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.061 *
Number of reflections	21889 *
Completeness [%]	87.9 *
Redundancy	6.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	291	PEG-2000, magnesium sulfate, ethanol, Tris buffer, CDP, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.0 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	EDTA	0.1 (mM)
6	1	reservoir	PEG2000	16 (%)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir		10 (mM)
9	1	reservoir	dithiothreitol	1 (mM)